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Clustal Omega 1.2.4_1

By default, only a fraction of the distance matrix is calculated, for speed. Calculate full matrix may be more accurate under some circumstances but is slower and needs more memory.

Redo the alignment multiple times to improve accuracy. Both the HMM and the guide tree will be recalculated each time.

Normally, if iteration is specified, a new guide tree and HMM will be calculated for each iteration. Use this option to restrict the number of iterations for either.